BIOANALYSIS &
STEROID ENDOCRINOLOGY

making a new beginning in 2017

Advanced analytical assays of steroid hormones
based on chromatography-mass spectrometry provide
the quantitative steroid signatures in various
physiological events.

We are looking for excellent graduate students as well as postdoc fellows.

Research Interests


  • 확대보기
    Old Techniques, but New Looks Gas chromatography-based separation of 84 endogenous steroids coupled to single-quadrupole mass spectrometric detection, as the screening assay, was firstly reported in 2007. Then, many specific assays for sterols, progestins/corticoids, androgens, and estrogens have been developed with better analytical sensitivity as well as selectivity using GC-MS/MS, LC-SIM/MS, and LC-MS/MS combined with chemical derivatization methods. The analytical platforms can quantify corresponding steroid hormones from various biological specimens, such as blood, urine, frozen tissue, blood spot, saliva, and hair fiber. FIND OUT MORE > Mass Spectrom. Rev., 2015

  • 확대보기
    Translational Steroidomics Due to their biochemical roles in the endocrine system, quantitative evaluation of endogenous steroids helps understanding of both allostatic load and metabolic homeostasis in various physiological conditions. Our study focuses on explaining the activities of enzymes correlated with whole steroidogenesis; enzyme activity, which is represented by metabolic ratio between precursor and metabolite, may be
    a useful tool for clinical diagnosis as well as a mining biomarker. A novel aspect of this study is the introduction of the “metabolic signature,” which expresses different enzyme activity related adrenal and hypertensive diseases as well as neurodegenerative disorders. quantify corresponding steroid hormones from various biological specimens, such as blood, urine, frozen tissue, blood spot, saliva, and hair fiber. FIND OUT MORE > BMB Reports, 2015 J. Steroid Biochem. Mol. Biol., 2015

  • 확대보기
    Mitochondrial Steroidogenesis In addition to many metabolic enzymes, including 3β-HSD and 11β-hydroxylase, mitochondria in the steroidogenic cells contain the cholesterol side-chain cleavage enzyme (P450scc), which converts cholesterol to pregnenolone and is responsible for steroidogenic regulation in the adrenal, gonad, placenta and brain. Even before this step, the access of cholesterol to the mitochondria is regulated by the StAR protein. Due to human deficiency diseases and mitochondrial morphology have been described for both StAR and P450scc, many endocrine diseases and reproductive
    events, characterized by lipid accumulation and metabolic malfunction, may be elucidated by steroid signatures. such as blood, urine, frozen tissue, blood spot, saliva, and hair fiber. FIND OUT MORE > Mol. Cell. Endocrinol., 2017

  • 확대보기
    Diagnostic Tools in Clinical Practice Dried blood spot and hair fiber, as the non-invasive biological specimens, increase the accessibility of clinical screening test in remote area, and they are relatively easy to handle and preserve with no specific conditions. As with a previous result in sitosterolemia, which is a inherited lipid metabolic disorder with accumulation of plant sterols in the blood vessel, we are eager in developing the patient-friendly
    assays after clinical findings in biomarker studies to bring experimental techniques into clinical practice. In contrast, a slice of frozen tissue section provides the local levels of steroids to enhance our understanding of the pathophysiological significances as the alternative diagnostics. ccumulation and metabolic malfunction, may be elucidated by steroid signatures. such as blood, urine, frozen tissue, blood spot, saliva, and hair fiber. FIND OUT MORE > Endocr-Relat. Cancer, 2016